In the animation following the "People falling out of tall buildings" sketch, when the caterpillar walks into his house and climbs into the bed the door to the bedroom is still open. Once the caterpillar falls asleep and the camera fades out, when the camera fades back into him, the door is now closed.
During the Dead Parrot sketch, John Cleese's arms alternate between crossed and on his hips between shots.
During the mountaineer sketch, Eric Idle clearly breaks character and suppresses laughter when John Cleese reads from the dictionary.
Camera reflected on one of the granny's motorcycle license plates.
On the sign advertising Edward Ross's (Graham Chapman) appearance on "It's the Arts", the phrase "This Weeks Guest" is missing an apostrophe (i.e. "This Week's Guest").