When David S. Goyer first pitched the idea of doing a Blade movie, the executives of New Line felt there were only three actors who could possibly do the role: Wesley Snipes, Denzel Washington and Laurence Fishburne, but in Goyer's mind, Snipes was always the perfect choice for the character of Blade.
(at around 1h 45 mins) While discussing the character of Deacon Frost, Wesley Snipes described him as the kind of guy who'd ice-skate uphill. Stephen Norrington and David S. Goyer loved the phrase so much they worked it into the film.
David S. Goyer explained in the DVD commentary that the scene where Karen touches Blade's sword was originally longer as she went on to discover a weird, hybrid infant of some kind floating in a tank. It came with a jump scare, and Goyer says "I think it would have scared the living shit out of the audience, but New Line felt it was just too horrible."
The scene where Quinn (Donal Logue) attacks Karen in the hospital corridor features lots of screaming, but they knew something was wrong when Logue started yelling too. During the tussle, he fell face first onto the hard floor and completely dislodged his jaw. He had broken it in an accident years prior, and opening his mouth too wide and too fast can unhinge it. They were filming in an abandoned hospital but had to rush to a real one, "but I've got this guy who's dressed as a third-degree burn victim, essentially naked, running in with his jaw hanging down." The room cleared out pretty fast.
Kris Kristofferson's character, Whistler, was created for Blade's cameo on Spider-Man: The Animated Series (1994). He was liked so much by Marvel's CEO that he was adopted into the Marvel universe.
Jeff Imada: (at around 52 mins) Martial artist/stuntman has a cameo at the vampire archives where he and several other familiars incapacitate Blade.
Stephen Norrington: (at around 40 mins) During the chase with Officer Krieger, the vampire on the side of the road biting the girl's neck. In the alternate ending on the DVD, the blue figure in the black coat on the roof is Norrington again, as an unnamed vampire.