According to Bruce Timm, "It wasn't intentional, but the Venus Fly-trap creature looks like a vagina with teeth. Originally, it looked like Audrey II, and I said, 'Naww, let's not do that, what other kind of plant can we do? What if it's like a big snow-peapod?' I started sketching it out, and stopped when I realized what it looked like, but it worked. In a way, it's a very good visual metaphor for what she is, a man-killer."
The perfume advertised through Ivy's cosmetics firm is called "Nightshade", which is named after a branch of a plant family containing several poisonous plants.
In the comics, Poison Ivy originally had a natural immunity to all poisons and toxins which explains why she could wear the poison lipstick in this episode with no effect to her. Eventually, her constant use of poison and toxins permanently affected her body chemistry so that she could naturally poison others with a touch or kiss. She couldn't always control the poison her body secreted but as a dangerous psychopath, she didn't care.
For the scene where Poison Ivy slinks out of a restaurant, and all the men turn to watch her go, the Sunrise animators could not animate her derriere to look as seductive as the producers wanted. The task finally fell to storyboard artist Chen-Yi Chang, who animated the sequence at Warner Bros with cels shipped overseas for Sunrise to film.
First appearance of Poison Ivy and Renee Montoya.