When Batman enters the door marked "Riddle???", the question marks turn into guns and fire projectiles at him, knocking him off his feet and blasting off the door. A few frames later, Batman gets to his feet and runs to exit the room and closes the door that had just been blown off its hinges.
When the Riddler first creates his virtual chessboard, it has seven rows and at least ten files, rather than the normal eight and eight. Even after the board is fixed to contain the normal amount of squares, while the white king and queen are positioned correctly (the white queen should be on the player's left), the board is not. Each player's back row should start with a black square on the left and end with a white square on the right.
At 16:57 When in VR, Batman rides a winged horse past the constellation of Orion with Orion's Belt consisting of 4 stars.
There are actually only 3: Alnitak, Alnilam, and Mintaka.