When Doctor Octopus forces Peter Parker to play sick, he wears a white suit and tie. This is just like his look in the early 90's comics where he also wore a white suit including the "Return of the Sinister Six" storyline.
Doctor Octopus unmasking a weak Peter Parker after an unsatisfying and then trying to lure the "real" Spider-Man to a fight is adapted from "The Amazing Spider-Man" (Vol. 1) #12.
This episode is based on "The Amazing Spider-Man" (Vol. 1) Annual #1, the first appearance of the Sinister Six.