3 weeks before this episode aired, Neil Patrick Harris (Barney) came out, and in this episode his character is against his gay brother getting married.
Coincidentally, Wayne Brady, who plays Barney's gay brother, is straight while Neil Patrick Harris, who plays (straight) Barney, is gay.
James Stinson (Wayne Brady), wears the same tie - purple with purple flower - when introduced in the apartment - that Barney Stinson (Neil Patrick Harris) wore in Season 2, Episode 5 "World's Greatest Couple" when he decides to stay in for the night with Lily.
Sawyer Fox who played Barney's nephew Eli is the son of How I Met Your Mother producer Rob A. Fox. In the last scene when Barney is hitting on one of James' old girlfriends the shot of Eli smiling at him was something they were worried wouldn't happen because he had just gotten up from a nap.
Marshall (Jason Segel) is seen wearing a t-shirt from Notre Dame University, when it is a known fact that Ted, Lilly, and Marshall attended Weslyen University