Aquaman and the Wonder Twins do not appear in this episode. Superman mentions they left for Atlantis, but left Gleek behind.
While fighting the first monster created by Dr Frankenstein, Robin says "Alright you green gargantuan, you asked for it!". This is a reference to the 1966 film, War of the Gargantuans. The The film features Gaira, the green gargantuan, battling Sanda, the brown gargantuan. This is the sequel to Frankenstein Conquers The World, the debut of Sanda as a creature created from an immortal human heart taken from Dr Frankenstein's lab during WW2, and exposed to the nuclear bombing of Hiroshima.