The weather-based villain Gizmoduck fights in the first scene bears a striking resemblance to Dr. Heinz Doofenshmirtz, a villain from the animated Disney Show Phineas and Ferb (2007).
First appearance of Gandra Dee in the DuckTales (2017), who was a character originally created for the original DuckTales (1987). In the original series, Gandra Dee was portrayed as Fenton Crackshell's love interest and eventual girlfriend, which was intended as a parody of the superhero romance theme such as with Superman and Lois Lane. Also in the original series she was a receptionist instead of a scientist.
One of the TV Broadcasts provide a message saying "Once again the day is saved, thanks to Gizmoduck". This is a reference to the closing monologue to each episode of the Cartoon Network series The Powerpuff Girls (1998), and the News Anchor to use that line was even voiced by that show's interim narrator Tom Kenny.
Gandra exclaims "Ada Lovelace!" when she sees Fenton's invention. Lovelace was a real world scientist who is widely regarded as one of the earliest pioneers in the field that eventually became computer sciences.
Fenton's list of "People who know I'm Gizmoduck" consists of: Gyro, Launchpad, Dewey, Huey , Mark) Beaks, Scrooge, The mailman, The dry cleaner, That barista at Starducks, Everyone who lives on my block, Webby