This is the first episode in the Diamond & Pearl series where Dawn's Piplup does not appear.
Brock catches a Croagunk.
This episode marks the first time sparkles appear whenever a Pokémon gets released from its Poké Ball.
Close to the end of the episode, Meowth says "Besides, there's three minutes left!", thus breaking the fourth wall by acknowledging the remaining time in the episode.
Jessie's Dustox is revealed to know Stun Spore.