The crow that is seen in the pilot is in reference to Damon, who in the books had the power to transform into a crow. The producers said they didn't run with that because they didn't want to give him too many powers too quickly and leave no room for growth.
This is the only episode shot in Vancouver, the rest of the show has been filmed in Georgia.
During this episode, "Never Say Never" by The Fray plays. Candace (Accola) King goes on to marry Joe King from The Fray.
In the book Elena has blonde hair, but in show she has brown.
An unnamed acoustic theme used throughout Kyle XY(Often at emotional climaxes) is used when Stefan and Elena are talking on the bridge.
This is probably because both Brian Young(Executive Producer/Writer on The Vampire Diaries) and Julie Plec(Executive Producer/Writer on The Vampire Diaries) worked on Kyle XY together as writers and producers.