Lady Sif from Thor (2011) and Thor: The Dark World (2013) makes her second appearance in the series.
A Crossfire-Maximum Paintball arcade game is present in the SHIELD base. This game was released by Team Play in 2005 and is considered hard to find by collectors.
During their training session, Skye asks May if she wants her to go full "Mortal Kombat" on her. Ming-Na Wen actually played fighting game character Chun-Li in the live action movie Street Fighter (1994). The Street Fighter video game is similar to - and came out around the same time as - the original Mortal Kombat game.
Jaimie Alexander the actor who plays Lady Sif also plays Jane Doe in Blindspot (2015) a woman who also loses her memory.